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KMID : 0525720140190020130
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Journal of Chitin and Chitosan
2014 Volume.19 No. 2 p.130 ~ p.137
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Physicochemical Properties and Antioxidant Activities of Polysaccharides from Styela plicata by Extraction Conditions
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Lee Dae-Hoon
Hong Joo-Heon
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Abstract
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In this study, we extracted polysaccharides from Styela plicata, and investigated to determine its physicochemical properties and antioxidant activities. The yields of hot water extract (SHE), autoclave extract (SAE), and enzyme extract (SEE) were 8.6, 10.4, and 11.9%, respectively. The uronic acid contents were 10.86 g/100 g (SEE), 6.13 g/100 g (SAE) and 3.12 g/100 g (SHE), respectively. The hexosamine and sulfate-glycosaminoglycans contents of SEE was highest of 21.40 g/100 g, 33.78 g/100 g respectively. Composition of amino acids was mainly leucine, valine, serine and threonine. With regard to mineral content, P was the highest in all samples, followed by Ca>Mg>K, and Fe contents. The spectrum of FT-IR showed wavenumber at 3,300~3,400 cm-1, 2,900 cm-1, 1,650 cm-1, and 820~850 cm-1 representing the polysaccharide. The decomposition temperature was in the range of 300 and showed relatively good enough for their thermal stabilities. The oxygen radical absorbance capacity (ORAC), FRAP activity and ferrous ion chelating effect of SEE were 215.38 μmole TE/g, 295.38 μM, and 27.79%, respectively. Electron donating abilities, reducing power and tyrosinase inhibition activity increased with an increase in extract concentration.
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KEYWORD
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Styela plicata, Polysaccharide, Extraction condition, Physicochemical property, Antioxidant activity
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